Sadism on the Screen
BMJ (Clinical Research Edition)
cases of large adrenal cortical tumours which showed no liormonal activity, says: " Fuchsinophil staining by Broster and Vines's (1933) method gave for the most part negative results, ailthough occasional granules scattered through the cells of the tumours took up the red stain feebly." This wording suggests uncertainty about the results of the method. Some time ago I tried this method on an adrenal gland, not a tumour, from a case of virilism, so that a positive result was to be expected.
... o be expected. However, the results obtained by the method described by Broster and Vines' were indefinite; I found it impossible to reproduce the appearances shown in their illustrations and in a section, in the possession of one of my colleagues, which Professor Vines was believed to have stained. In my preparations red-staining granules were seen in the cells of the zona reticulata, but they stained neither very strongly nor much more strongly than many other structures. Moreover, the cells of the cortex generally stained red or pink, instead of the blue shade shown in the pictures and sections mentioned. After many experiments I found it was possible to get the desired results by " post-chroming " for 24-48 hours at 370 C., staining in ponceau-fuchsin for 5-10 minutes, differentiating as described by Broster and Vines, and then prolonging the time in aniline blue until the cortical cells lost their residual pink or red and became blue or purplish. This might require 30 minutes or more. In this way strongly fuchsinophil granules could be demonstrated in abundance in many of the cells of the zona reticulata, but few or none in the corresponding cells of a number of control cases.