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RP-UPLC method development and validation for the simultaneous estimation of proguanil and atovaquone in pharmaceutical dosage form
Journal of Chemical and Pharmaceutical Research
A simple, rapid, and accurate reversed phase ultra-performance liquid chromatographic (RP-UPLC) method has been developed and subsequently validated for the simultaneous determination of proguanil (PRG) and Atovaquone (ATV) in combination. The separation is carried out using a mobile phase consisting of 0.05% perchloric acid and Acetonitrile. The column used is Extended C18(50 × 3.0 mm, 1.8 µm). With flow rate of 0.8 mL/min using UV detection at 257nm. The total run time is 6 min and thefatcat:7g32u4hjjnczrdp5witpllywpu