Second National Youth Conference "Biological sciences for a better future

Jaspreet Patti, Iliyana Stefanova, Milena Draganova-Filipova, Plamen Zagorchev, Plamen Zagorchev
2015 J. BioSci. Biotechnol   unpublished
Rosemary oil is an essential oil extracted from Rosemary officinalis. It consists of more than 50 compounds with different biological activities. Empirically it is used in therapy for many years. Rosemary oil is analyzed in vivo and in vitro as many of its effects-antimicrobial, antitumor, antioxidant etc. are known. We cannot find suggested reports concerning the Rosemary oil effect on contractility of striated muscles. The aim of this study was to investigate the influence of Rosemary oil on
more » ... of Rosemary oil on maximal tetanic contraction provoked by electrical field stimulation. We use abdominal transversal muscles strips isolated from guinea pigs. The tetanic muscles force, was provoked by means of repeated multipulse electrical field stimulation, square-wave pulses of supramaximal intensity (60V) and 0.5 ms in duration were applied at frequency of 50 Hz for 3s followed by 7s pause. The concentration effect curve of the action of Rosemary oil (1.5.10-6 M÷1.5.10-3 M) on this type electrical field stimulated-muscle activity was plotted on a statistical graph. During our observation the effect of Rosemary oil on contractile properties of abdominal muscles and calculated half maximal effective concentration (EC50). Our study revealed that myorelaxant activity of the essential oil in this concentration is time-dependent and after application in the interval of 25 minutes the contractile ability is decreased to 5.5% within comparison with the initial one. Our investigation is a first report on an action of Rosemary oil on the guinea pig's striated muscles strips.