Intrauterine bacterial inoculation and level of dietary methionine alter amino acid metabolism in nulliparous yearling ewes1

T. M. Thelen, C. A. Löest, J. B. Taylor, S. Wang, G. S. Lewis
2007 Journal of Animal Science  
Using an intrauterine bacterial inoculation method, our objective was to determine the effects of acute sepsis and level of dietary metabolizable Met (MM) on splanchnic metabolism of AA in ewes. Twenty-four nulliparous yearling Rambouillet-cross ewes (initial BW = 65.1 ± 0.6 kg), surgically fitted with chronic-indwelling catheters in hepatic and portal veins, a mesenteric vein and artery, and the uterine lumen, were assigned to a 2 × 2 factorial arrangement of treatments. Factors were
more » ... ne bacterial inoculation (noninoculated vs. inoculated) and level of MM [low (2.28 g/d) vs. high (3.91 g/d)]. Beginning 12 h before sampling, inoculated and noninoculated ewes received 10-mL intrauterine infusions of Escherichia coli (9.69 × 10 11 cfu) + Arcanobacterium pyogenes (2.76 × 10 12 cfu) and of sterile saline, respectively. Uterine infection was induced in ewes that received intrauterine bacterial
doi:10.2527/jas.2006-851 pmid:17785602 fatcat:2q5x2r5mrzegvpaw6qeth7i6om