MPK6 Kinase Regulates Plasma Membrane H+-ATPase Activity in Cold Acclimation

Ilian Giordano Ponce-Pineda, Laura Carmona-Salazar, Mariana Saucedo-García, Dora Cano-Ramírez, Francisco Morales-Cedillo, Araceli Peña-Moral, Ángel Arturo Guevara-García, Sobeida Sánchez-Nieto, Marina Gavilanes-Ruíz
2021 International Journal of Molecular Sciences  
Cold and freezing stresses severely affect plant growth, development, and survival rate. Some plant species have evolved a process known as cold acclimation, in which plants exposed to temperatures above 0 °C trigger biochemical and physiological changes to survive freezing. During this response, several signaling events are mediated by transducers, such as mitogen activated protein kinase (MAPK) cascades. Plasma membrane H+-ATPase is a key enzyme for the plant cell life under regular and
more » ... conditions. Using wild type and mpk3 and mpk6 knock out mutants in Arabidopsis thaliana, we explored the transcriptional, translational, and 14-3-3 protein regulation of the plasma membrane H+-ATPase activity under the acclimation process. The kinetic analysis revealed a differential profiling of the H+-ATPase activity depending on the presence or absence of MPK3 or MPK6 under non-acclimated or acclimated conditions. Negative regulation of the plasma membrane H+-ATPase activity was found to be exerted by MPK3 in non-acclimated conditions and by MPK6 in acclimated conditions, describing a novel form of regulation of this master ATPase. The MPK6 regulation involved changes in plasma membrane fluidity. Moreover, our results indicated that MPK6 is a critical regulator in the process of cold acclimation that leads to freezing tolerance and further survival.
doi:10.3390/ijms22126338 fatcat:cfgi7gfsdrgytcnsza3r47qvoq