Effects of mulberry heartwood extract on genes related to lipid metabolism

Milena Pasheva, Milka Nashar, Oskan Tasinov, Diana Ivanova
2015 Scripta Scientifica Pharmaceutica  
Scientific evidence exists that extracts from different parts of Morus nigra (mulberry tree), mainly fruits, leaves and root bark, exhibit various beneficial effects, such as antiviral, antihyperglycemic, antiatherogenic and hypotensive activity. Although it is known that the heartwood has a specific phytochemical composition, the biological effects of this part of the plant are not yet clarified. This study examines the effect of 40% ethanol infusion from mulberry heartwood on the expression
more » ... on the expression of three genes related to lipid metabolism: CCAAT/enhancer binding protein-alpha (C/EBPα) gene, adipogenic transcriptional factors peroxisome proliferator-activated receptor gamma (PPARγ) and fatty acid-binding protein (aP2) genes in adipocytes. Results obtained present that treatment with two concentrations of mulberry ethanol extract decreased expression of aP2 messenger RNA as compared to controls. Based on these results we concluded that heartwood mulberry extract had beneficial effects on lipid metabolism and could be a potential source for search of active compounds for treatment of metabolic disorders related to adipose tissue metabolism. Chang WC, et al. Mulberry water extracts inhibit rabbit atherosclerosis through stimulation of vascular smooth muscle cell apoptosis via activating p53 and regulating both intrinsic and extrinsic pathways. J Agric Food Chem. 2014;62(22):5092-5101. 5. Chan KC, Yang MY, Lin MC, Lee YJ, Chang WC, Wang CJ. Mulberry leaf extract inhibits the development of atherosclerosis in cholesterol-fed rabbits and in cultured aortic vascular smooth muscle cells. J Agric Food Chem. 2013;61(11):2780-2788. 6. Choi BH, Ahn IS, Kim YH, Park JW, Lee SY, Hyun CK, Do MS Berberine reduces the expression of adipogenic enzymes and inflammatory molecules of 3T3-L1 adipocyte. Exp Mol Med. 2006;38:599-605. 7. Cristancho AG, Lazar MA. Forming functional fat: a growing understanding of adipocyte differentiation. Nat Rev Mol Cell Biol. 2011;28:722-34. 8. De Rosso MD, Cancian D, Panighe, A, Vedova AD, Flamini R. Chemical compounds released from five different woods used to make barrels for aging wines and spirits: volatile compounds and polyphenols. Wood Sci Tech. 2009;43:375-385. 9. DeFuria J, Bennett G, Strissel KJ, Perfield JW 2nd, Milbury PE, Greenberg AS, et al.. Dietary blueberry attenuates whole-body insulin resistance in high fat-fed mice by reducing adipocyte death and its inflammatory sequelae. J Nut. 2009;139:1510-1516.
doi:10.14748/ssp.v1i1.1084 fatcat:2u6c4lzdnnbcblidwxohh4vjuq