The Human Skin Barrier Is Organized as Stacked Bilayers of Fully Extended Ceramides with Cholesterol Molecules Associated with the Ceramide Sphingoid Moiety

Ichiro Iwai, HongMei Han, Lianne den Hollander, Stina Svensson, Lars-Göran Öfverstedt, Jamshed Anwar, Jonathan Brewer, Maria Bloksgaard, Aurelie Laloeuf, Daniel Nosek, Sergej Masich, Luis A. Bagatolli (+2 others)
2012 Journal of Investigative Dermatology  
The skin barrier is fundamental to terrestrial life and its evolution; it upholds homeostasis and protects against the environment. Skin barrier capacity is controlled by lipids that fill the extracellular space of the skin's surface layer-the stratum corneum. Here we report on the determination of the molecular organization of the skin's lipid matrix in situ, in its near-native state, using a methodological approach combining very high magnification cryoelectron microscopy (EM) of vitreous
more » ... section defocus series, molecular modeling, and EM simulation. The lipids are organized in an arrangement not previously described in a biological system-stacked bilayers of fully extended ceramides (CERs) with cholesterol molecules associated with the CER sphingoid moiety. This arrangement rationalizes the skin's low permeability toward water and toward hydrophilic and lipophilic substances, as well as the skin barrier's robustness toward hydration and dehydration, environmental temperature and pressure changes, stretching, compression, bending, and shearing. These authors contributed equally to this work.
doi:10.1038/jid.2012.43 pmid:22534876 fatcat:fuyflhzjuzesvo4ys6owxuy4ju