UVC irradiation suppresses platelet-derived growth factor-BB-induced migration in human pancreatic cancer cells

JUNJI KAWAGUCHI, SEIJI ADACHI, ICHIRO YASUDA, TAKAHIRO YAMAUCHI, TAKASHI YOSHIOKA, MASAHIKO ITANI, OSAMU KOZAWA, HISATAKA MORIWAKI
2011 Oncology Reports  
We have recently reported that short wavelength ultraviolet-C (UVC) irradiation inhibits cell growth and induces apoptosis in human pancreatic cancer cells. In this study, we investigated the effect of UVC on platelet-derived growth factor (PDGF)-BB-induced migration in pancreatic cancer cells, AsPC1 and BxPC3. In cell migration assays using a Boyden chamber Transwell, PDGF-BB exerted a maximum effect on migration of these cells at a dose of 70 ng/ml after 36 h of treatment. PDGF-BB also caused
more » ... phosphorylation of p44/p42 mitogen-activated protein kinase (MAPK), stress-activated protein kinase/c-Jun-N-terminal kinase (SAPK/JNK) and Akt, but not of p38 MAPK in these cells. Pretreatment of these cells with UVC at a dose over 10 J markedly suppressed PDGF-BB-induced migration. Since UVC significantly inhibited PDGF-BB-induced phosphorylation of Akt, and subsequent glycogen synthase kinase (GSK) 3β, but not p44/p42 MAPK and SAPK/JNK, it is likely that UVC inhibits PDGF-BB-induced migration by suppressing the Akt-GSK3β pathway in pancreatic cancer cells. Taken together with our previous findings, UVC could be a useful tool for the treatment of patients with pancreatic cancer.
doi:10.3892/or.2011.1612 pmid:22200892 pmcid:PMC3583433 fatcat:gpc3lbcowrer3gogq24qa3jcb4