Multicomponent analysis of tumor-bearing animal urine by High Performance Liquid Chromatography

Noriko YAMADA, Chiho YASUDA, Yasuhisa SAKURAI, Nobuharu TAKAI
1988 Journal of Life Support Technology  
In first attempt to detect the metabolic change in various diseases from multicomponent urine analysis by HPLC,we investigated the differences in chromatogram between tumor-bearing and normal mouse urine.Animals received by intraperitoneal injection of the S-180sarcoma(ascites type),and their urine were collected with development of cancer.We analyzed normal and tumor bearing mouse urines using reverse type of gel column(LS-410),under the eluent condition of stepwise system of phosphate
more » ... cetonitrile.We obtained reproducible chromatogram, exhibiting approximately90peaks within60min.We observed considerable change in chromatogram pattern with development of cancer.
doi:10.5136/lifesupport1986.2.165 fatcat:5garcemq6ffvtey6odwrwo2lai