Oscillatory fluid flow elicits changes in morphology, cytoskeleton and integrin-associated molecules in MLO-Y4 cells, but not in MC3T3-E1 cells

Huiyun Xu, Jian Zhang, Jiawei Wu, Ying Guan, Yuanyuan Weng, Peng Shang
2012 Biological Research  
Interstitial fl uid fl ow stress is one of the most important mechanical stimulations of bone cells under physiological conditions. Osteocytes and osteoblasts act as primary mechanosensors within bones, and in vitro are able to respond to fl uid shear stress, both morphologically and functionally. However, there is little information about the response of integrin-associated molecules using both osteoblasts and osteocytes. In this study, we investigated the changes in response to 2 hours of
more » ... e to 2 hours of oscillatory fl uid fl ow stress in the MLO-Y4 osteocyte-like cell line and the MC3T3-E1 osteoblast-like cell line. MLO-Y4 cells exhibited a signifi cant increase in the expression of integrin-associated molecules, including OPN, CD44, vinculin and integrin αvβ3. However, there was no or limited increase observed in MC3T3-E1 osteoblast-like cells. Cell area and fi ber stress formation were also markedly promoted by fl uid fl ow only in MLO-Y4 cells. But the numbers of processes per cell remain unaff ected in both cell lines.
doi:10.4067/s0716-97602012000200008 pmid:23096360 fatcat:gwnpof56ujerxpqly3mvarjjc4