Distinct localization of GLUT-1, -3, and -5 in human monocyte-derived macrophages: effects of cell activation

Daniela Malide, Theresa M. Davies-Hill, Mark Levine, Ian A. Simpson
1998 American Journal of Physiology. Endocrinology and Metabolism  
Malide, Daniela, Theresa M. Davies-Hill, Mark Levine, and Ian A. Simpson. Distinct localization of GLUT-1, -3, and -5 in human monocyte-derived macrophages: effects of cell activation. Am. J. Physiol. 274 (Endocrinol. Metab. 37): E516-E526, 1998.-We determined subcellular localization of GLUT-1, GLUT-3, and GLUT-5 as human monocytes differentiate into macrophages in culture, and effects of the activating agents N-formyl-methionyl-leucylphenylalanine (fMLP) and phorbol myristate acetate (PMA).
more » ... stern blot analysis demonstrated progressively increased GLUT-1, rapidly decreased GLUT-3, and a delayed increase of GLUT-5 expression during differentiation. Confocal microscopy revealed that each isoform displayed a unique subcellular distribution and cell-activation response. GLUT-1 was localized primarily to the cell surface but was also detected in the perinuclear region in a pattern characteristic of recycling endosomes. GLUT-3 exhibited predominantly a distinct vesicle-like staining but was present only in monocytes. GLUT-5 was found primarily at the cell surface but was detectable intracellularly. Activation with fMLP induced similar GLUT-1 and GLUT-5 redistributions from intracellular compartments toward the cell surface. PMA elicited a similar translocation of GLUT-1, but GLUT-5 was redistributed from the plasma membrane to a distinct intracellular compartment that appeared connected to the cell surface. These results suggest specific subcellular targeting of each transporter isoform and differential regulation of their trafficking pathways in cultured macrophages.
doi:10.1152/ajpendo.1998.274.3.e516 fatcat:p2xrlau53ne2lpaf6i4sfljwjm