Bioimaging assessment and effect of skin wound healing using bone-marrow-derived mesenchymal stromal cells with the artificial dermis in diabetic rats

Hirokazu Inoue, Takashi Murakami, Takashi Ajiki, Mayumi Hara, Yuichi Hoshino, Eiji Kobayashi
2008 Journal of Biomedical Optics  
We investigate the relationship between the fate and healing effect of transplanted mesenchymal stromal cells ͑MSCs͒ in a rat diabetic skin wound model. Rats are treated with streptozotocin to induce diabetic conditions. A full-thickness skin defect is surgically made on the head of diabetic rats, and covered with an artificial dermis impregnated with either bone marrow cells ͑BMCs͒ or bonemarrow-derived MSCs from firefly luciferase ͑luc͒ transgenic ͑Tg͒ rats. Wound healing is evaluated using
more » ... animetry and immunohistochemistry, and the fate of transplanted MSCs is determined using in-vivo luminescent imaging. The diabetic wound treated with MSCsimpregnated artificial dermis is significantly smaller than that treated with artificial dermis alone at 1 week postoperation. Photons of luc+ MSCs are detected at the transplanted site during healing ͑3 weeks͒, whereas those of luc+ MSCs are depleted only after 1 week postimplantation. Immunohistochemistry at the healing site treated with MSCs demonstrates that CD31+ vessels increase with expression of vascular endothelial growth factor, suggesting that MSCs accelerate angiogenesis. These findings suggest that transplanted MSCs could be retained at wound sites during the healing process in a diabetic rat model, and subsequently promote wound healing through angiogenesis.
doi:10.1117/1.3042266 pmid:19123682 fatcat:y76f7obl25fo5ps5czs2jzprve