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The synthesis and resolution of a class of chiral organic fluorophores, axially chiral 4,4-difluoro-4-bora-3a,4a-diaza-s-indacenes (Ax*-BODIPY), is described. Ax*-BODIPYs were prepared through a modular synthesis, resolved by preparative chiral HPLC and absolute stereochemistry assigned by ECD.doi:10.1039/c4cc00851k pmid:24676233 fatcat:gsbgfmqde5cn3d24q6lvtp66vy