Chemical composition and SDS-PAGE analysis of radiated, extruded and sprouted soybean
Research Opinions in Animal and Veterinary Sciences
The aim of the present study was to examine the effects of gamma irradiation and extrusion on chemical composition and protein subunit patterns of soybean (SB), sprouted soybean (SSB) and extruded soybean (EBM) using SDS-PAGE electrophoresis. Part of the soybeans was germinated in 3 days in a pollution-free environment. After extracting fat, samples were poured in extraction solution and stored at temperature of -20°C for electrophoresis. Ash decreased significantly (P<0.05) in soybean meal
... in soybean meal radiated with 45kGy. Ether extract (EE) was reduced significantly (P<0.05) in soybean meal irradiated with 15kGy and sprouted soybean irradiated with dose 15 kGy. Neutral detergent fibre (NDF) decreased significantly (P<0.05) in soybean irradiated with dose 30 kGy and soybean meal irradiated with dose 30 kGy. Acid detergent fibre (ADF) decreased significantly (P<0.05) in soybean irradiated with dose 30 kGy and 45 kGy. Acid detergent lignin (ADL) decreased significantly (P<0.05) in soybean irradiated with dose 45 kGy and extruded sprouted soybean. Regarding SDS-PAGE electrophoresis results, SB, SSB and EBM proteins were mainly composed of two major polypeptides including Glycinin and β-Conglycinin and the subunit molecular weights (MWs) were affected by the processing. The results of the study showed that the extrusion had the greatest impact on reducing Glycinin subunits and dose of 15 and 45 kGy of the gamma irradiation reduced and increased MWs of the protein subunits, respectively. Gamma irradiation cross-linking the subunit of proteins which this characterize can be a good substitute for SB, SSB and EBM digestion and absorption To cite this article: Moghaddam M, P Shawrang, AA Sadeghi and M Chamani, 2016. Chemical composition and SDS-PAGE analysis of radiated, extruded and sprouted soybean. Res. Opin. Anim. Vet. Sci., 6(3): 101-107.