The Validity of Marker-assisted Selection Using Sex Linked SCAR Markers in Kiwifruit (Actinidia chinensis cv 'Rainbow Red') Seedlings
DNAマーカーを利用したキウイフルーツ'レインボーレッド'由来の実生集団における雌雄判別

Satoru MURAKAMI, Hideyuki KATAI, Shinya YAMADA, Motohiro TANEISHI
2015 Shokubutsu Kankyo Kogaku  
Marker-assisted selection (MAS) using four (SmX, SmY, SmY1, and SmY2) sequence characterized amplified region (SCAR) markers linked to the sex determining locus were performed in Actinidia chinensis (cv 'Rainbow-Red'). The relationship between the SCAR markers and the sex was investigated. No SmX signal was detected, and SmY, SmY1, and SmY2 were mainly detected in males. Regarding the sex determination precision of each marker in 182 'Rainbow-Red' seedlings, SmY1 was 95 %, SmY was 77 %, and
more » ... Y was 77 %, and SmY2 was 68 %. Further, we investigated the existence of these markers in twenty six A. chinensis, A.deliciosa, and A.rufa varieties. These markers might not be detected in several varieties, and is believed to improve the efficiency of kiwifruit breeding programs. Especially SmY1 has great potential in enhancing kiwifruit breeding programs using 'Rainbow-Red' as the breeding material.
doi:10.2525/shita.27.68 fatcat:zxhjxtztkzeohkjvwvmbixnupa