Protein Biomarker Validation: A Mass Spectrometry Approach

M Walid Qoronfleh
2017 Journal of Proteomics & Bioinformatics  
This concept communication describes a mass spectrometry workflow specific to biomarker assay development and validation. The primary objective of this workflow is to significantly decrease the cost and timeline for assay development and biomarker validation. The biomarker development workflow can be separated into five development stages that take less than 6 months to complete. Figure 5: Absolute quantitation assay scheme. Panel A) Typical chromatographic data for native peptides and their
more » ... ptides and their respective isotope labeled partners. Panel B) Calibration data for a six point curve with analytical duplicate measurements. Panel C) Absolute quantitation data for a protein.
doi:10.4172/jpb.1000448 fatcat:npa4moxwqbfudlooostpwyg74q