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Development of a New Epitope Tag Recognized by a Monoclonal Antibody to Rickettsia typhi
2001
BioTechniques
The epitope recognized by a mouse monoclonal antibody (MAb) to the crystalline surface layer protein of Rickettsia typhi , SRT10, was mapped to 10 amino acid residues (SRTag TFIGAIATDT). The oligonucleotide sequence covering the epitope recognized by SRT10 was inserted into a mammalian expression vector together with multiple cloning sites. When the SRTag was fused in frame to the coding region of the NCC27/CLIC1 gene and expressed in mammalian cells, the MAb SRT10 could detect the tagged
doi:10.2144/01313st08
pmid:11570498
fatcat:3e56bwxce5ht7ktq2p4qygfkfu