Production of proteases from organic wastes by solid-state fermentation: downstream and zero waste strategies

Maria Marín, Adriana Artola, Antoni Sánchez
2018 3 Biotech  
24 Production of enzymes through solid-state fermentation (SSF) of agro-industrial wastes 25 reports high productivity with low investment. The extraction of the final product from 26 the solid waste and solid disposal represent the main cost of the process. In this work, 27 the complete downstream processes of SSF of two industrial residues for the production 28 of proteases, soy fibre (SF) and a mixture of hair and sludge (HS), were studied in terms 29 of activity recovery, using different
more » ... raction parameters (extracting solvent, ratio 30 solid:solvent and extraction mode). Activity after lyophilisation was tested. Solid waste 31 valorisation after extraction was studied using respiration techniques and biogas 32 production tests, as part of a zero waste strategy. 33 Results showed a maximum extraction yield of 91% for SF and 121% for HS, both in 34 agitated mode and distilled water as extraction agent. An average activity recovery of 35 95 ± 6 % and 94 ± 6 % for SF and HS respectively was obtained after lyophilisation and 36 redissolution. To reduce the cost of extraction, a ratio 1:3 w: v solid-solvent in static 37 mode is advised for SF, and 1:2 w:v extraction ratio in agitated mode for HS, both with 38 distilled water as extracting agent. Both composting and anaerobic digestion are suitable 39 techniques for valorisation of the waste material. 40 41
doi:10.1007/s13205-018-1226-y pmid:29607286 fatcat:en2ljt57mngoriifbidf7bq3yq