Genetic surveillance in the Greater Mekong Subregion and South Asia to support malaria control and elimination [article]

Christopher Jacob, Nguyen Thuy-Nhien, Mayfong Mayxay, Richard Maude, Huynh Hong Quang, Bouasy Hongvanthong, Viengxay Viengxay, Thang Ngo Duc, Huy Rekol, Rob van der Pluijm, Lorenz Von Seidlein, Rick Fairhurst (+84 others)
2020 medRxiv   pre-print
The use of parasite genetic data by National Malaria Control Programmes (NMCPs) is currently limited, and typically focused on specific genetic features or a small number of study sites. We have developed GenRe-Mekong, a platform for genetic surveillance of malaria in the Greater Mekong Subregion (GMS). By integrating simple sample collection procedures in the routine operations of public health facilities, GenRe-Mekong enables NMCPs to conduct large-scale surveillance project in endemic
more » ... t in endemic regions. Samples are processed by the SpotMalaria platform, which uses high-throughput technologies to produce a broad set of genotypes, including most known drug resistance markers, species markers and a genomic barcode. Through the application of heuristics based on published evidence, GenRe-Mekong delivers Genetic Report Cards, a compendium of genotypes and phenotype predictions that are used to map prevalence of resistance to multiple drugs. To date, GenRe-Mekong has worked with NMCPs in five countries, and with several large-scale research projects, processing 9,645 samples from clinical cases. The monitoring of resistance markers has been especially valuable for NMCPs tracking the recent rapid spread of DHA-piperaquine resistant parasites across the region. In Vietnam and Laos, data from GenRe-Mekong have provided novel knowledge about the spread of these resistant strains in provinces previously thought to be unaffected. GenRe-Mekong facilitates data sharing by aggregating at regional level results from different countries, providing cross-border views of the spread of resistant strains.
doi:10.1101/2020.07.23.20159624 fatcat:mg7tube4yvblhdttrbsfaglaw4