Detection of Five Rash-Associated Viruses Using Multiplex Real-Time PCR during 2006^|^ndash;2011

Atsushi Kaida, Hideyuki Kubo, Jun-ichiro Sekiguchi, Minori Ohyama, Kaoru Goto, Atsushi Hase, Nobuhiro Iritani
2012 Japanese journal of infectious diseases (Print)  
Many viruses have been reported to be associated with rash development. Multiplex realtime PCR was used to investigate the presence of 5 viruses associated with rashes: measles virus (MV), rubella virus (RV), human parvovirus B19 (PVB19), human herpes virus 6 (HHV-6), and HHV-7. A total of 187 clinical specimens from 169 patients with erythema were collected between January 2006 and December 2011. Virus-positive specimens were as follows: MV (n = 23), PVB19 (n = 8), RV (n = 2), HHV-6 (n = 5),
more » ... V-7 (n = 1), MV and PVB19 (n = 1), and HHV-6 and HHV-7 (n = 1). All of the MV-positive specimens were collected in 2007 and the strains whose sequence were available (21/24, 87.5z) were of genotype D5. The results indicate that multiplex real-time PCR might be a useful screening method for detecting and differentiating rash-associated viruses in clinical specimens.
doi:10.7883/yoken.65.430 fatcat:cqonrarym5a4pe6kvmpsecazya