Differential Pulse Polarographic Determination of Clopidogrel Bisulphate in Pure and Pharmaceutical Dosage Forms Using Dropping Mercury Electrode
International Journal of Current Research in Chemistry and Pharmaceutical Sciences
Differential pulse polarographic analysis (DPPA) of Clopidogrel Bisulphate (CLPB) in pure and pharmaceutical dosage forms usi ng drop mercury electrode (DME) has been studied. Various parameters (electrolyte, pH, pulse time, pulse amplitude, etc.) affecting on the CLPB determination were examined. The best definition of the analytical signals was found in sodium acetate 0.02 M buf fer at pH 8.0. Under the optimum conditions, liner calibration graph, Ip=f(CCLP), was obtained in the concentration
... ranges of 5x10 -7 M to 3x10 -5 M (0.1609 to 9.6546 μg.mL -1 of CLP), at -1275 to -1310 mV (versus Ag/AgCl) with percent relative standard deviations (RSD%) did not exceed 2.9% for the concentrations 0.1609 μg.mL -1 of CLP. Regression analysis showed a good correlation coefficient (R 2 =0.9997) between Ip and concentration over the mentioned range. The limit of detection (LOD) and the limit of quantification (LOQ) were to be 0.0160 and 0.0485 µg.mL -1 , respectively. The proposed method was validated for linearity, precision and accuracy (LOD and LOQ), repeatability, sensitivity, robustness and specificity. The developed method is applicable for the determination of CLP in pure and different dosage forms in presence of aspirin with average recovery of 99.9 to 101.8% and the results are in good agreement with those obtained by the HPLC reference method.