Accessibility of Extracellular Space in the Rhabdome of the Living Isolated Retina of the Crayfish

W. Schröder, H. Stieve, I. Classen-Linke
1979 Zeitschrift für Naturforschung C - A Journal of Biosciences  
Diffusion of the tracer enzyme horseradish peroxidase (HRP) into the extracellular spaces of living isolated crayfish retinas was monitored by light-and electronmicroscopic identification of the reaction product obtained after immobilisation of the enzyme by fixation. During the incubation in enzyme containing saline (up to 3 hours) the extracellularly recorded electroretinogram (ERG) stayed normal and showed no substantial change. The reaction product of the enzyme HRP was found only
more » ... ound only extracellularly in the space between the microvilli in the rhabdome. The results indicate an exchange of substances via extracellular pathways between the extra­ cellular side of the photosensory membrane and the bathing fluid in the living isolated crayfish retina.
doi:10.1515/znc-1979-1-227 fatcat:esltq4sip5behd3nvaewiapawu