Photodynamische Inaktivierung von Mikroorganismen mittels Perinaphthenonderivaten - Einfluss einer LED405nm-Lampe

Patrick Bauer
The treatment of fungal infections with aPDT is an alternative to conventional antibiotics, since there are only a few drugs available for treating fungal infections. Two of these drugs are Imidazol and Miconazol, but there are an increasing number of candida-strains, which are resistant against this sort of drugs. Hence, the photodynamic inactivation of C. albicans with photosensitizers, which previously showed an antimicrobial effect against bacteria, was investigated. Therefore, the first
more » ... efore, the first part of the dissertation examines whether several new derivatives of SAPyR have a photodynamic effect on C. albicans. For that reason, different experiments with C. albicans and derivatives of SAPyR dependent on several parameters like incubation time, concentration of the photosensitizers, solvent, light dose and washing of the suspension were performed. Therefore derivatives of SAPyR containing different length of alkyl-chains were investigated. Thus it could be demonstrated for the first time that an increase of the antimicrobial photodynamic inactivation of C. albicans was dependent on the length of the alkyl-chains of these new perinaphthenone derivatives. In addition by using uptake experiments it was examined whether the alkyl-chains of the photosensitizers influence the binding or absorption of the photosensitizers by C. albicans. Again, it was found that the alkyl-chains have a positive impact on the "uptake" of Candida. The second part of the dissertation investigates the impact of a LED405nm lamp in comparison to the BlueV UV802L on the photodynamic inactivation of S. aureus and E. coli using the photosensitizer SAPyR. Therefore, the microorganisms were incubated for 10s with different photosensitizer concentrations and were radiated with an energy dose of 1,2 J/cm² using both lamps. In order to get a better comparison of both lamps, the absorbed photons were calculated. Having a look at the photodynamic inactivation of the bacteria, no difference between the two lamps was observed. Though, with the LED405nm lamp, [...]
doi:10.5283/epub.34680 fatcat:efq6kdhyxrghfiu7hebm4fnape