Energy transduction in Escherichia coli: physiological and biochemical effects of mutation in the uncB locus

S M Hasan, T Tsuchiya, B P Rosen
1978 Journal of Bacteriology  
The transduction of energy through biological membranes was investigated in Escherichia coli strains defective in the ATP synthetase complex. Everted vesicles prepared from strains containing an uncA or uncB mutation were compared with those of the parental strain for their ability to couple energy derived from the oxidation of substrates by the electron transport chain or from the hydrolysis of ATP by the Mg2e-adenosine triphosphatase, as measured by the energy-dependent quenching ofquinacrine
more » ... fluorescence or the active transport of 'Ca2e. Removal of the Mg2e-adenosine triphosphatase from membranes derived from the parental or an uncA strain caused a loss of energy-linked functions and a concomitant increase in the permeability of the membrane for protons. Proton impermeability was restored by treatment with N,N'-dicyclohexylcarbodiimide. When membranes of the uncB strain were treated in a similar manner, there was no loss of respiratory-driven functions, nor was there a change in proton permeability. These observations suggest that the uncB mutation specifically results in alteration of an intrinsic membrane protein portion of the ATP synthetase complex, leading to the loss of the proton channel necessary for the generation or utilization of the electrochemical gradient of protons by that complex. Loss of the function of the proton channel is believed to prevent the transduction of energy through the ATP synthetase complex. Escherichia coli mutants with alterations in the ATP synthetase complex or BFOF1 have been isolated by a number of laboratories (2, 3, 17, 20) . (FOF1 refers to the proton-translocating and N,N'-dicyclohexylcarbodiimide [DCCD]-
doi:10.1128/jb.133.1.108-113.1978 fatcat:w5klqudxsra23npv4wfhh6qwga