Acute toxicological studies of leaf extracts of Morus indica L. in rats

P. Vanitha Reddy, N. Sreenivas, Asna Urooj
2016 Annals of Phytomedicine An International Journal  
Morus indica L. is a potent source of phytochemicals and its antioxidant activity at in vitro and ex vivo level is very well studied. Further to proceed with the animal studies, the safety and efficacy of the different solvent extracts has to be evaluated at maximum dosage, i.e., 2 g/kg body weight (BW) as per Organisation for Economic Co-operation and Development (OECD) guidelines.The acute toxicity study recorded no mortality or any toxic reaction in any group after 14 d. of extra cts
more » ... ration at 2 g/kg BW. The extracts aqueous-MAq; dechlorophylised-MDc; 80% methanol-M8M did not cause any behavioral or physical changes in experimental rats. There was no significant (p  0.05) difference in the biochemical parameters, analysed between the groups. Slight elevation in activity of alanine transaminase (ALT) and alkaline phosphatase (ALP) in MDc treated groups was observed, but did not exert any deleterious effect on the normal metabolism which was supported by the histopathology of liver. Extracts did not induce any oxidative stress which is indicated by, no significant difference (p  0.05) in the control and experimental lipid peroxides (LPO) and glutathione values. The serum total cholesterol and triglycerides were less than the control group. Histopathological studies showed no remarkable changes in hepatocytes after 14 d. of oral administration of MAq, MDc and M8M extracts. The study contributes in establishing the non-toxic quality parameters of M. indica leaf and the results indicate the non toxic effect of the extracts.
doi:10.21276/ap.2016.5.2.14 fatcat:acjob6ldvfhazdzyiaiu6lq6be