Alterations of aqueous humour outflow following argon laser trabeculoplasty in monkeys

S. Melamed, D. L. Epstein
1987 British Journal of Ophthalmology  
We performed argon laser trabeculoplasty in one eye in each of three cynomolgus monkeys, leaving the contralateral eyes as controls. Four weeks later in both eyes of each monkey we infused cationised ferritin as a tracer of the aqueous outflow pathways for 30-40 minutes before fixation. In the control eyes cationised ferritin was found throughout the conventional aqueous outflow tract, labelling trabecular endothelial cells, both inner and outer walls of Schlemm's canal, and the collecting
more » ... els. No permeation of cationised ferritin through continuous cellular layers such as the corneal endothelium was detected. In the lasered spots of the treated eyes cationised ferritin was found to label the cellular sheet covering the trabecular scar at the lasered site, but no tracer was detected within the scar or in Schlemm's canal immediately beneath. In contrast in adjacent non-lasered regions the trabecular cells, the observed juxtacanalicular herniations, and the vacuoles of the inner wall as well as Schlemm's canal itself were extensively labelled. Our findings suggest impermeability to aqueous humour flow through the actual lasered lesion, with shift of flow to adjacent, non-lasered regions. In the normal monkey these regions compensate structurally and functionally in ways that may help to deal with this diverted flow. on 30 April 2019 by guest. Protected by copyright. Br J Ophthalmol: first published as 10.1136/bjo.71.10.776 on 1 October 1987. Downloaded from
doi:10.1136/bjo.71.10.776 pmid:3676149 pmcid:PMC1041305 fatcat:lx2qn5tvsrg3zmaztra62ruedy