Immune profiling with a Salmonella Typhi antigen microarray identifies new diagnostic biomarkers of human typhoid

Li Liang, Silvia Juarez, Tran Vu Thieu Nga, Sarah Dunstan, Rie Nakajima-Sasaki, D. Huw Davies, Stephen McSorley, Stephen Baker, Philip L. Felgner
<span title="2013-01-09">2013</span> <i title="Springer Nature"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/tnqhc2x2aneavcd3gx5h7mswhm" style="color: black;">Scientific Reports</a> </i> &nbsp;
Current serological diagnostic assays for typhoid fever are based on detecting antibodies against Salmonella LPS or flagellum, resulting in a high false-positive rate. Here we used a protein microarray containing 2,724 Salmonella enterica serovar Typhi antigens (.63% of proteome) and identified antibodies against 16 IgG antigens and 77 IgM antigens that were differentially reactive among acute typhoid patients and healthy controls. The IgG target antigens produced a sensitivity of 97% and
more &raquo; ... icity of 80%, whereas the IgM target antigens produced 97% and 91% sensitivity and specificity, respectively. Our analyses indicated certain features such as membrane association, secretion, and protein expression were significant enriching features of the reactive antigens. About 72% of the serodiagnostic antigens were within the top 25% of the ranked antigen list using a Naïve bayes classifier. These data provide an important resource for improved diagnostics, therapeutics and vaccine development against an important human pathogen.
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1038/srep01043">doi:10.1038/srep01043</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/23304434">pmid:23304434</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC3540400/">pmcid:PMC3540400</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/xivfd52tmjdp7itqyp7s6xjzya">fatcat:xivfd52tmjdp7itqyp7s6xjzya</a> </span>
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