Possible activation of murine T lymphocyte through CD98 is independent of interleukin 2/interleukin 2 receptor system

Hiroshi KOMADA, Akiyo IMAI, Emi HATTORI, Morihiro ITO, Hideki TSUMURA, Toshiko ONODA, Mariko KURAMOCHI, Machiko TANI, Kanako YAMAMOTO, Mizuho YAMANE, Mitsuo KAWANO, Machiko NISHIO (+6 others)
2006 Biomedical research  
CD98 is a widely expressed cell surface heterodimetric protein of 125 kDa. Its expression is upregulated during lymphocyte activation induced by mitogen, superantigen, conventional antigen, and a combination of phorbol myristate acetate (PMA) and ionomycin. However, the role of CD98 in the immune system is not so well understood. The role of CD98 in murine T lymphocyte proliferation was investigated, especially in correlation with the interleukin 2 (IL-2)/interleukin 2 receptor (IL-2R) system.
more » ... onoclonal antibody (mAb) directed against murine CD98 heavy chain (mCD98HC) suppressed the proliferation of lymphocytes stimulated with concanavalin A (Con A). Anti-mCD98HC mAb did not suppress the expression of IL-2Rα. Anti-IL-2Rα mAb, which suppressed DNA synthesis, did not inhibit the expression of CD98HC. Murine IL-2 (recombinant), which induced considerable DNA synthesis by lymphocytes stimulated with a sub-optimal dose of Con A, did not induce CD98HC expression in lymphocytes. In addition, anti-mCD98HC mAb did not inhibit the production of IL-2 by lymphocyte stimulated with Con A. Taken together with these findings, it was speculated that the CD98 system is independent of the IL-2/IL-2R system in murine T lymphocyte activation.
doi:10.2220/biomedres.27.61 pmid:16707844 fatcat:gi2gqdfe4vg4hm5aflxcn5ia3m