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The standard deviation and relative standard deviation of measurements in the refractive index detection of liquid chromatography are predicted based on the 1/f fluctuation model which is made up of white noise and the Markov process. First, repeated measurements and Monte Carlo simulation corroborate the reliability of this prediction in the absence of flow pulsation. Pulsation, if any, can be distinguished in the frequency space from the 1/f noise which is common to most analyticaldoi:10.2116/analsci.15.549 fatcat:4bevr3zfh5hrzpwqhcbgg3ggiq