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A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells
2015
Cell Reports
Graphical Abstract Highlights d Streamlined affinity purification of protein complexes using CRISPR/Cas9 and TALENs d Purification of native holoenzymes regulated under physiological conditions d Blueprint for the systematic and unbiased mapping of the human protein interactome SUMMARY Conventional affinity purification followed by mass spectrometry (AP-MS) analysis is a broadly applicable method used to decipher molecular interaction networks and infer protein function. However, it is
doi:10.1016/j.celrep.2015.09.009
pmid:26456817
fatcat:khxrbpnpgvbs3efxy6nabuz2ga