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Differences in the mycelium growth of several Trichoderma pleurotum and T. pleuroticola isolates and their biotic interaction with three Pleurotus florida strains were determined. The examined T. pleurotum and T. pleuroticola isolates differed with respect to their mycelium growth rates on the PDA substrate as well as their biotic impact on P. florida. Trichoderma pleuroticola isolates restricted the growth of P. florida mycelium more than T. pleurotum isolates. The examined P. florida strainsfatcat:toy5onumhzev7aq3gdz5glhgla