Two-dimensional analysis of proteins sedimenting with simian virus 40 chromosomes

B I Milavetz, L D Spotila, R Thomas, J A Huberman
1980 Journal of Virology  
The nonhistone proteins sedimenting in low-salt glycerol gradients with simian virus 40 chromosomes were analyzed by two-dimensional gel electrophoresis, utilizing nonequilibrium pH gradients as the first dimension and sodium dodecyl sulfate-gel electrophoresis as the second dimension. By densitometric quantitation of the radiolabeled proteins present in each fraction of the gradients, it was possible to identify proteins sedimenting with all or a fraction of the simian virus 40 chromosomes.
more » ... 1 sedimented with simian virus 40 chromosomes; additional evidence for its binding to chromosomes was obtained by immunochemical techniques. Four proteins (Mr 25,000, pI 6.0; Mr 32,000, pI 7.2; Mr 35,000, pl 8.5; and Mr 80,000, pl 7.2) sedimented with specific subsets of chromosomes. 854 on May 9, 2020 by guest
doi:10.1128/jvi.35.3.854-864.1980 fatcat:d6my23h7zfglzhfcq3kxapmevq