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A simple, rapid, specific and accurate RP-HPLC method was developed and validated for the estimation of venlafaxine hydrochloride in pharmaceutical dosage form. The method involved an isocratic elution of venlafaxine hydrochloride in ODS C18 column using mobile phase consists of methanol : ortho-phosphoric acid (90:10 v/v). The wavelength of detection was 225 nm. The method showed good linearity in the range of 7.5-37.5 μg/mL with correlation coefficient of 0.9981. The retention time was 2.41doi:10.5281/zenodo.4491918 fatcat:ie2bz4bmhjfflctsmn6itif6wy