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A Cost-effective Amino-acid-type Selective Isotope Labeling of Proteins Expressed inLeishmania tarentolae
2009
Journal of Biomolecular Structure and Dynamics
We report a cost efficient approach for amino-acid-type selective isotope labeling of proteins expressed in Leishmania tarentolae. The method provides an economically advantageous alternative to recently established protocol for isotopic labeling using expensive synthetic media. The method is based on cultivation of the L. tarentolae expression strain in a cheap complex medium supplemented with labeled amino acid(s). In this protocol, a labeled amino acid is deliberately diluted in the medium
doi:10.1080/07391102.2009.10507287
pmid:19385703
fatcat:u52orul6z5eetiu4jn6vjsyhiy