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Among the challenges faced by diesel engines combustion nowadays are to reduce emission especially Nitrogen Oxide (NOx) and Particular Matter (PM) while enhancing fuel efficiency and power. The purpose of this review is to explore the mixture formation of biodiesel combustion using constant volume chamber and optical visualization. This paper will review the development of a single-shot combustion system and constant volume chamber. An overview of the relation of mixture formation anddoi:10.4028/www.scientific.net/amm.773-774.555 fatcat:ac5wjign7nbpnghsv4d37w2tra