Introduction a Novel Poly-Epitope Vaccine Against Mycobacterium tuberculosis Infection; an Immunoinformatics Approach

Ehsan Rashidian, Ali Foroharmehr, Amin Jaydari
2020 International Journal of Infection  
Tuberculosis is known as one of the most dangerous diseases caused by Mycobacterium tuberculosis. Although there are different strategies to prevent tuberculosis, it is still considered terminal in the world. Objectives: The current study aimed to design a novel poly-epitope vaccine based on three antigenic proteins against tuberculosis. Methods: First, we selected the antigenic proteins of Mycobacterium tuberculosis such as Dnak, FbpA, and katG from the database to make a poly-epitope vaccine.
more » ... ly-epitope vaccine. Then, we predicted B cell, MHCI, and MHCII epitopes of the antigenic proteins via reliable online tools. We applied the best-epitopes to assemble a poly-epitope vaccine. Also, we evaluated physicochemical features, the antigenicity of the whole vaccine, and protein structures of the designed poly-epitope vaccine via the most precise tools. Finally, we adapted the coding DNA sequence of the vaccine to be expressed in the prokaryotic system and cloned it theoretically in the pET32a (+) vector. Results: The results showed that the molecular weight and length of the designed poly-epitope vaccine were 32 kDa and 308 amino acids, respectively. The protein structure results demonstrated that the designed poly-epitope vaccine contained 19.48% alpha-helix and 73.05% random coil. These results showed that 92.2% of amino acid residues were located in the favored region. Finally, it was clarified that the antigenicity of the designed poly-epitope vaccine was 12333. Conclusions: According to the results of the current project, it seems that the designed poly epitope vaccine can be an appropriate candidate to control tuberculosis.
doi:10.5812/iji.110423 fatcat:dbhrcbgpdrekfmpbwsc3hi367u