The importance and challenges of in vivo-like enzyme kinetics

Karen van Eunen, Barbara M. Bakker
2014 Perspectives in Science  
Realistic mathematical models of biochemical pathways require kinetic data that have been measured under physiological conditions. However, until recently most enzyme-kinetic data were obtained under conditions that yield maximum activity rather than physiological activity. The implementation of such non-physiological kinetic data is an important source of discrepancies between model simulations and corresponding experimental data. Here we review a number of initiatives to develop media for
more » ... me-kinetic assays that resemble the intracellular conditions of various organisms. We will discuss the challenges faced when developing such media, but also illustrate their relevance, since in vivo-like kinetic data improve the predictive value of kinetic models substantially.
doi:10.1016/j.pisc.2014.02.011 fatcat:odcqkb4xkzhlre2wzjgrnzmx4a