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The importance and challenges of in vivo-like enzyme kinetics
Perspectives in Science
Realistic mathematical models of biochemical pathways require kinetic data that have been measured under physiological conditions. However, until recently most enzyme-kinetic data were obtained under conditions that yield maximum activity rather than physiological activity. The implementation of such non-physiological kinetic data is an important source of discrepancies between model simulations and corresponding experimental data. Here we review a number of initiatives to develop media fordoi:10.1016/j.pisc.2014.02.011 fatcat:odcqkb4xkzhlre2wzjgrnzmx4a