Molecular Characterization and Phylogenetic Analysis of the Eukaryotic Translation Initiation Factor 4A Gene in Antheraea pernyi (Lipdoptera: Saturniidae)

Miao-Miao Chen, Yan-Qun Liu, Yan Li, Rui Yao, Mo Chen, Run-Xi Xia, Qun Li, Li Qin
2014 Journal of Insect Science  
Eukaryotic initiation factor 4A (eIF-4A) is an essential component for protein translation in eukaryotes. The eIF-4A gene (ApeIF-4A) was isolated and characterized from Antheraea pernyi (Guérin-Méneville) (Lepidoptera: Saturniidae). The obtained cDNA sequence was 1,435-bp long with an open reading frame of 1,266 bp encoding 421 amino acids. The predicted amino acid sequence shared several conserved features as found in known eIF-4As and revealed 74 and 78% identities with eIF-4As of Homo
more » ... L. and Drosophila melanogaster (Meigen), respectively. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that ApeIF-4A was transcribed at four developmental stages and in all tissues tested, suggesting that it plays an important role in development of A. pernyi. Homologous alignment suggested that eIF-4As are highly conserved throughout evolution of eukaryote organisms. Phylogenetic trees based on the amino acid and nucleotide sequences of eIF-4A demonstrated a similar topology with the classical systematics, suggesting that it has the potential value in phylogenetic inference of eukaryotes.
doi:10.1093/jisesa/ieu030 pmid:25480968 pmcid:PMC5633915 fatcat:b62h6dcwq5ee7kfx4gb5kfbsda