Nuclei DNA Slide: A Novel Method to Measure Biological Effects of Radiation Damage
Journal of Psychology & Psychotherapy
Purpose: Although there are many physical and chemical radiation detectors, biological effects of radiation damage are hard to measure. So we aim to use a novel in vitro method to measure biological effects of radiation. Materials and methods: Acellular DNA, nuclei DNA fixed on slides, is used in our experiment. The nuclei DNA slides used as a model are radiated for 0, 5, 25 and 45 min with Ultraviolet (UV) lamp. The DNA damage of nuclei DNA slides was detected with comet assay, and the
... ay, and the pictures of comet assay were measured by comet auto-analysis software-Comet A1.0, and Differences among groups are compared with SPSS 11.0. Results: When nuclei DNA slides are radiated with UV, the DNA damage is significantly, also there is timeresponse relationship between UV and DNA damage. Conclusions: The nuclei DNA slides can be used to measure biological effects of radiation, and the method is sensitive and specific to detect biological effects of radiation. Rad, USA), are concentration-adjusted to 1 × 10 6 cells/ml, and are sandwiched between a lower layer of 1% (wt/vol) normal-meltingpoint agarose (Bio-Rad, USA) and an upper layer of 1% (wt/vol) lowmelting-point agarose on slides. The slides are immersed in cell lysis solution (BIO-LAB, CHINA) for 2 hours at 4°C. Then, the full nuclei DNA slides are prepared, which can be stored at 4°C in saturated humidity for 2 months. UV radiation procedure For UVA radiation, three UV lamps with an emission wavelength peak at 365 nm were used to ra¬diate the nuclei DNA slides. The slides were dunked in buffer on ice and placed below the lamps about 20 cm, exposed for 0, 5, 25 and 45 min, there were two slides in every time point.