Nicotiana glauca (N. glauca) (Solanaceae) is an ornamental plant. It has insecticidal properties. However, these uses cannot compensate for the overall negative impacts of this plant. This study was aimed to evaluate the larvicidal effect of the aqueous extract of N. glauca leaves on the 4th stage larva of Culiseta longiareolata (Cs. longireolata). N. glauca leaves were harvested, dried and powdered using an electric mill. Hot aqueous extraction was done with 150 g powdered leaf in 1000

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... led water. The dry extract was partitioned in various beakerswith 20 larvae, and the three concentrations 14.85 g / l, 25.66 g / l and 62.4 g / l were determined. The preliminary results showed that used extract has a high efficiency compared to natural substances of plant or microbial origin. This efficiency is expressed by the calculated toxicological parameters which are successively LC50 and LC90, with 26.87 and 55.14g/l, As for lethal times 0.46 and 1 days respectively. N. glauca can be considered among the plants with important insecticidal effects in biological control against mosquitoes. Preliminary toxicity tests on Cs. longireolata have confirmed its toxicity for these vectors.