L-Threonine dehydrogenase from goat liver. Feedback inhibition by methylglyoxal

M Ray, S Ray
1985 Journal of Biological Chemistry  
L-Threonine dehydrogenase, which forms aminoacetone from L-threonine and NAD, has been extensively purified from goat liver. A feedback inhibition of this enzyme has been observed with methylglyoxal. Kinetic data and other experiments indicate that methylglyoxal acts at a site other than the active site of the enzyme. The enzyme contains a single subunit of Mr 89,000. The apparent Km values of the enzyme for L-threonine and NAD were found to be 5.5 and 1 mM, respectively.
pmid:3888978 fatcat:sb4tovd6bfeqrc3mckuilz4d7a