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The effects of proline on in vitro proliferation and propagation of doubled haploid sugar beet (Beta vulgaris)
Turkish Journal of Botany
Doubled haploid induction is one of the available methods normally used for sugar beet breeding. Gynogenic haploid explants of sugar beet induced with 1 or 2 mg L -1 6-benzylaminopurine were treated with 5 g L -1 colchicine, then subcultured on a solidified MS medium plus 0.2 mg L -1 kinetin. Colchicine doubled the chromosome number of 27.7% of the treated haploid explants. With the aim of increasing the number of doubled haploid explants, the effects of five levels of proline (0.0, 0.1, 0.2,doi:10.3906/bot-1709-14 fatcat:su7udpkjzvg57ia4dgwn2zpq7q