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The Potential Role of Proteoglycans in Cationic Lipid-mediated Gene Delivery
Journal of Biological Chemistry
Recent evidence supports a role for proteoglycans in polycation-mediated gene delivery. Therefore, the interaction of glycosaminoglycans with cationic lipid-DNA complexes (CLDCs) has been characterized using a combination of biophysical approaches. At low ionic strength, CLDCs bind to heparin-derivatized Sepharose particles, with the ratio of cationic lipid to DNA controlling the binding. Incorporation of the helper lipids cholesterol or 1,2-dioleoyl-phosphatidylethanolamine increases thedoi:10.1074/jbc.m007940200 pmid:11443107 fatcat:3rvwi3dfmrfvrmhnfzfoq2doiy