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Abstracts Accepted for Publication
Objectives: To investigate the effect of macrophage conditioned media on fibroblast activation. Methods: PBMC-derived macrophages from healthy control (n=3 females, mean age 50.8±21.9 years) and diffuse scleroderma (n=4 females, mean age 54.8±15.7 years, mean disease duration 73.2±90.3 months, 2 with antiScl70 and 2 with antiRNA polymerase antibodies) individuals were cultured in RPMI/10% FBS/M-CSF (4ng/ml)/P/S, quiesced in media with 1% BSA replacing the FBS, and left untreated (M0) or treateddoi:10.1136/annrheumdis-2017-eular.5310 fatcat:lpojhpt3qfh4pdyymbrggubzvu