Chitosan-Based Heparan Sulfate Mimetics Promote Epidermal Formation in a Human Organotypic Skin Model

Brooke L. Farrugia, Yaolei Mi, Ha Na Kim, John M. Whitelock, Shenda M. Baker, William P. Wiesmann, Zhe Li, Peter Maitz, Megan S. Lord
2018 Advanced Functional Materials  
Biomimetic materials that replicate biological functions have great promise for use as therapeutics in regenerative medicine applications. Heparan sulfate (HS) is the natural binding partner for growth factors enabling longer half-lives and potentiation of their signaling. In this study a water soluble chitosan-arginine was modified with sulfate moieties in order to mimic the structure of HS. Sulfated chitosan-arginine with a degree of sulfation of 58% bound fibroblast growth factor 2 with
more » ... r affinity than HS. The sulfated chitosan-arginine also promoted epithelial cell migration and supported the formation of an expanded epidermis in an organotypic skin model. Furthermore, sulfated chitosanarginine promoted the expression of the HS proteoglycan, perlecan, by both epithelial and fibroblast cells. Perlecan itself modulates the activity of mitogens and is essential for the formation of the epidermis. The synthesized sulfated Ch-Arg derivatives mimicked HS, supported formation of the epidermis and thus have the potential to assist in wound healing.
doi:10.1002/adfm.201802818 fatcat:iv5upvffdngvphat7nzvmh4f4m