Original Article Study on the clinical expressions of MMP-1, MMP-2 and CD14 protein in liver tissues of infants with biliary atresia

Chunmei Tian, Lin Zhang, Yifei Wu, Cuicui Wang, Yanyan Zhang, Shuxia Zhu
2018 Int J Clin Exp Med   unpublished
Objective: To observe and explore the clinical expressions of MMP-1, MMP-2 and CD14 protein in liver tissues with different grades of liver fibrosis in infants with biliary atresia. Methods: A total of 20 infants with liver fibrosis and biliary atresia treated in our hospital from January 2015 to June 2017 were selected as the experimental group, while ten infants without hepatobiliary system disease were enrolled as the control group. The expression levels of MMP-1, MMP-2 and CD14 proteins in
more » ... d CD14 proteins in liver tissues were detected by enzyme-linked immunosorbent assay (ELASA) and immunohistochemistry. Results: The expressions of MMP-2 and CD14 protein in liver tissues of grade I, II and III in experimental group were significantly higher than those in control group (P<0.05). There were no significant differences in MMP-1 expression of all grades and MMP-2 and CD14 protein expressions of grade IV between two groups (P>0.05). The positive expressions of MMP-1 and MMP-2 protein were positively correlated with the degree of liver fibrosis (r=0.53, P<0.05; r=0.57, P<0.05). The positive expression of CD14 protein were negatively correlated with the degree of liver fibrosis (r=-0.42, P<0.05). Besides, there was a positive correlation between the expression of MMP-1 and MMP-2 protein (r=0.67, P<0.05). In addition, CD14 was negatively correlated with MMP-1 and MMP-2 protein expressions (r=-0.38, P<0.05; r=-0.41, P<0.05). Conclusion: The formation of liver fibrosis in infants with biliary atresia was positively correlated with the expressions of MMP-1 and MMP-2, and negatively related to the expression of CD14 protein.
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