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Improved Plasmodium falciparum dilution cloning through efficient quantification of parasite numbers and c-SNARF detection
Background Molecular and genetic studies of blood-stage Plasmodium falciparum parasites require limiting dilution cloning and prolonged cultivation in microplates. The entire process is laborious and subject to errors due to inaccurate dilutions at the onset and failed detection of parasite growth in individual microplate wells. Methods To precisely control the number of parasites dispensed into each microplate well, parasitaemia and total cell counts were determined by flow cytometry usingdoi:10.1186/s12936-021-03816-w pmid:34162381 fatcat:n7ools2nbzfk7hvl4mjy3ppfeq