Cloning and polymorphism analysis of the 2-methyl-6-phytyl-1,4-benzoquinol methyltransferase gene (VTE3) in Arachis hypogaea, A. duranensis, and A. ipaënsis

F.Z. Liu, A.Q. Guo, Y.S. Wan
2013 Genetics and Molecular Research  
One of the critical enzymes involved in vitamin E biosynthesis in plants is 2-methyl-6-phytyl-1,4-benzoquinol methyltransferase (MPBQ MT). The full-length VTE3 cDNA (designated rVTE3-1 and -2) encoding MPBQ MT and the full-length DNA of VTE3 (designated gVTE3-1 and -2) were isolated from cultivated peanuts (Arachis hypogaea). The full-length DNA of VTE3 (designated gVTE3-A and -B) was isolated from the wild groundnut species A. duranensis (A-genome) and A. ipaënsis (B-genome), and polymorphism
more » ... , and polymorphism analysis of VTE3 was performed. The results demonstrated that rVTE3-1 and -2 both have a DNA sequence that is 1059 bp long and encodes 351 amino acids; the homology of the 2 amino acid sequences was 98.6%. The gVTE3-1 of cultivated Fenghua 2 peanut samples was 2710 bp long, with 3 introns located at 44-163, 772-1295, and 1603-2437 bp, and the Fenghua 2 gVTE3-2 was 2706 bp long, with 3 introns located at 1860 F.Z. Liu et al. ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (2): 1859-1871 (2013) 44-169, 778-1291, and 1599-2433 bp. The homology for gVTE3-1 and -2 across 13 cultivated peanut samples was 99.9 and 100%, respectively. gVTE3-1 and -2 were from A-and B-genome, respectively, with 96.6% homology between the 2 sequences. The present study demonstrated that abundant polymorphisms were present in the VTE3 genes from different genomes. Additionally, polymorphisms were observed in the gVTE3-1 alleles of the 13 cultivars and wild A. duranensis species but not in the gVTE3-2 alleles of the 13 cultivars and wild A. ipaënsis species.
doi:10.4238/2013.january.4.5 pmid:23315864 fatcat:r3cod5wd3rcnlp424xeuixsqyy