Fluid Resuscitation via Colon Alleviates Systemic Inflammation in Early Stage of Rats With Severe Acute Pancreatitis [post]

Tongtian Ni, Lili Xu, Silei Sun, Li Ma, Bing Zhao, Weijun Zhou, Yi Wen, Ning Ning, Erzhen Chen, Ying Chen, Enqiang Mao
2021 unpublished
Fluid Resuscitation Via Colon (FRVC) is a complementary therapy for severe acute pancreatitis (SAP) in early stage. The expression of intestinal dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) has been found to regulate systemic inflammation. The present study aimed to investigate the effect of FRVC on the expression of DC-SIGN in the colon tissue of SAP rats and its effect on the early response of systemic inflammatory and multiple organ injury. SAP
more » ... were induced by retrograding injection of sodium taurocholate into biliopancreatic duct. The expression of DC-SIGN was observed in the proximal and distal colons. Histological characteristics and inflammatory cytokines were examined to compare the effect of FRVC and intravenous fluid resuscitation (IVFR) treatment. The results showed that the expression of DC-SIGN in the proximal colon increased in a time-dependent manner in early stage of SAP rats. FRVC inhibits the expression of DC-SIGN in the proximal colon. Both FRVC and IVFR treatment alleviates histological injury of pancreas and colon. However, FRVC had an advantage over IVFR in alleviating lung injury and reducing Serum TNF-α, IL-6 and LPS. These results suggest that FRVC treatment might be helpful in suppressing systemic inflammation and preventing subsequent organ failure in early stage of SAP rats. The mechanism might be to inhibit the expression of DC-SIGN protein in the proximal colon.
doi:10.21203/rs.3.rs-146780/v1 fatcat:vc7rruuzrzb2zmccw2clt4yxhi