Articles of Significant Interest Selected from This Issue by the Editors

2007 Journal of Virology  
There are one major and two minor binding sites for eEF1A on the West Nile virus (WNV) genomic 3Ј-terminal stem-loop RNA. Davis et al. (p. 10172-10187) introduced mutations into the major binding site in a WNV infectious clone. Mutations that decreased eEF1A binding activity for the viral RNA in vitro decreased viral minus-strand RNA synthesis, whereas mutations that increased binding increased minus-strand synthesis. eEF1A coprecipitated and colocalized with flavivirus replication complex
more » ... cation complex proteins and bound to the 3Ј RNAs of divergent flaviviruses. These results support the functional relevance of the viral RNA-eEF1A interaction for flavivirus minus-strand RNA synthesis.
doi:10.1128/jvi.01609-07 fatcat:6p64q7lunzagdcbwzsomo55oi4